How does CRISPR-Cas9 achieve targeted gene editing in living cells?

30 Eki 11:00

I'm trying to wrap my head around the CRISPR-Cas9 system. Specifically, how does the Cas9 protein locate the correct DNA sequence and create a double‑strand break? What roles do the guide RNA and PAM sequence play in ensuring specificity? Also, could anyone outline the steps from DNA binding to repair pathway choice in a typical eukaryotic cell? Would love to hear explanations or analogies that helped you understand this process.